Rename samples flowjo 10
This spread sheet must have the following columns: Template formatįirst of all, we need to describe the gating hierarchy in a spread sheet (a plain text format). The flowWorkspace package provides the GatingSet as an efficient data structure to store, query and visualize the hierarchical gated data.īy taking advantage of these tools, the openCyto package can create the automated gating pipeline by a gatingTemplate, which is essentially the same kind of hierarchical gating scheme Automated GatingįlowCore, flowStats, flowClust and other packages provide many different gating methods toĭetect cell populations and draw gates automatically. We can achieve the same results by using the automated gating pipeline provided by this package. This is a gating scheme for a T cell panel, which tries to identify T cell sub-populations.